Fig 1: VEGFA signalling is involved in LINC02321-regulated bladder cancer progression. (A) Gene set enrichment analysis demonstrated that the expression of LINC02321 in The Cancer Genome Atlas bladder cancer dataset was associated with VEGFA signalling. (B) Pearson's correlation analysis demonstrated that LINC02321 expression correlated positively with VEGFA expression. (C) Immunohistochemical staining images from the Human Protein Atlas database demonstrated that the intensity of VEGFA staining in bladder cancer tissue was stronger compared with that in adjacent mucosa. LINC02321 regulated mRNA and protein expression levels of VEGFA, demonstrated using reverse transcription-quantitative PCR following transfection with (D) si-LINC02321 (*P<0.05 and ***P<0.001 vs. si-NC) and (E) OE-LINC02321 (***P<0.001 vs. vector) and (F) western blotting, respectively. (G) Survival time of patients with high LINC02321 expression levels was significantly shorter compared with patients with low LINC02321 expression levels. Data are presented as the mean ± standard deviation from three independent experiments. KEGG, Kyoto Encyclopedia of Genes and Genomes; LINC, long intergenic non-coding RNA; NC, negative control; NES, normalized enrichment score; OE, overexpression; si, small interfering RNA; TPM, transcripts per million.